Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kinetic studies on cyclophellitol analogues--mechanism-based inactivators

V W Tai1, P H Fung, Y S Wong

  • 1Department of Chemistry, Chinese University of Hong Kong, Shatin.

Biochemical and Biophysical Research Communications
|August 4, 1995
PubMed
Summary

Cyclophellitol diastereoisomers are potent irreversible inhibitors of alpha-D-glucosidase and alpha-D-mannosidase enzymes. These compounds effectively inactivate enzymes, with no reactivation observed after dialysis, highlighting their stability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Embryo-maternal communication mediated by extracellular vesicles in the early stages of embryonic development is modified by in vitro conditions.

Theriogenology·2023
Same author

2 Noninvasive method for bovine embryo sexing through the analysis of DNA content in extracellular vesicles.

Reproduction, fertility, and development·2022
Same author

50 The characteristics and microRNA content of extracellular vesicles are modulated by embryo developmental stage during preimplantation.

Reproduction, fertility, and development·2022
Same author

69&#x2003;DNase treatment of extracellular vesicles released by <i>in vitro</i>-produced bovine embryos increases accuracy of preimplantation genetic testing.

Reproduction, fertility, and development·2022
Same author

Comparison of modeling accuracy between Radixact<sup>®</sup>and CyberKnife<sup>®</sup>Synchrony<sup>®</sup>respiratory tracking system.

Biomedical physics & engineering express·2021
Same author

Using ultrasound for screening scoliosis to reduce unnecessary radiographic radiation - a prospective diagnostic accuracy study on 442 schoolchildren.

Studies in health technology and informatics·2021

Area of Science:

  • Biochemistry
  • Enzymology
  • Carbohydrate Chemistry

Background:

  • Alpha-D-glucosidase and alpha-D-mannosidase are crucial enzymes involved in carbohydrate metabolism.
  • Understanding enzyme inhibition mechanisms is vital for developing therapeutic agents and biochemical tools.
  • Cyclophellitol derivatives have shown potential as enzyme inhibitors.

Purpose of the Study:

  • To investigate the inhibitory effects of specific cyclophellitol diastereoisomers on alpha-D-glucosidase and alpha-D-mannosidase.
  • To characterize the kinetics and irreversibility of the enzyme inactivation process.

Main Methods:

  • Enzyme inhibition assays were performed using purified brewers yeast alpha-D-glucosidase and jack bean alpha-D-mannosidase.
  • Kinetic parameters (Ki, ki) for inactivation were determined.

Related Experiment Videos

  • Irreversibility was assessed by dialysis of enzyme-inhibitor complexes.

    • Main Results:

    • The (1R,6S)-cyclophellitol diastereoisomer effectively and irreversibly inactivated brewers yeast alpha-D-glucosidase (Ki = 26.9 µM, ki = 0.401 min⁻¹).
    • The (1R,2S,6S)-cyclophellitol diastereoisomer irreversibly inhibited jack bean alpha-D-mannosidase (Ki = 120 µM, ki = 2.85 min⁻¹).
    • Enzyme activity was not restored after dialysis, confirming irreversible inactivation.

    Conclusions:

    • Specific cyclophellitol diastereoisomers act as potent irreversible inhibitors of alpha-D-glucosidase and alpha-D-mannosidase.
    • These findings establish cyclophellitol derivatives as valuable tools for studying glycosidase function.
    • The irreversible nature of inhibition suggests potential therapeutic applications.