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Related Experiment Videos

Histone H3 transcript stability in alfalfa

T Kapros1, A J Robertson, J H Waterborg

  • 1Division of Cell Biology and Biophysics, University of Missouri-Kansas City 64110-2499, USA.

Plant Molecular Biology
|August 1, 1995
PubMed
Summary
This summary is machine-generated.

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Histone H3.1 mRNA in alfalfa is less stable than H3.2 mRNA, with shorter half-lives observed during non-S phase. This suggests translation impacts histone mRNA stability in plants, similar to animals.

Area of Science:

  • Molecular Biology
  • Plant Science
  • Genetics

Background:

  • Histones are crucial for DNA packaging and replication.
  • Histone gene expression is tightly regulated, particularly during the cell cycle.
  • Understanding histone mRNA stability is key to comprehending gene regulation.

Purpose of the Study:

  • To determine the stability of replication-dependent (H3.1) and -independent (H3.2) histone H3 transcripts in alfalfa.
  • To investigate the role of transcription and translation in regulating histone mRNA turnover.
  • To assess cell cycle-dependent changes in histone mRNA stability.

Main Methods:

  • Northern blot analysis was used to measure mRNA stability.
  • Inhibition of transcription (dactinomycin) and translation (cycloheximide) were employed.

Related Experiment Videos

  • Indirect assessment via histone protein synthesis was also performed.
  • Cell cycle synchronization and arrest techniques were utilized.

    • Main Results:

    • Replication-dependent histone H3.1 transcripts were approximately three-fold less stable than replacement variant H3.2 transcripts.
    • Half-lives of 2 hours for H3.1 and 7 hours for H3.2 mRNA were observed in actively growing cells.
    • Inhibition of translation significantly increased histone H3 mRNA levels.
    • H3.1 mRNA destabilization was detected in non-S phase cells.

    Conclusions:

    • Histone H3.1 mRNA is inherently less stable than H3.2 mRNA in alfalfa.
    • Histone mRNA turnover in plants appears to be dependent on translation, mirroring findings in animals.
    • Replication-dependent histone H3.1 mRNA stability is regulated throughout the cell cycle, being lower outside of S phase.