Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Morphological alterations caused by lithium in various cell lines

D P Matthopoulos1, M Tzaphlidou, I Leontiou

  • 1Laboratory of General Biology, Medical School, University of Ioannina, Greece.

Cell Biology International
|June 1, 1995
PubMed
Summary

Lithium exposure causes kidney cell damage, with higher concentrations leading to cell death. Non-kidney cells tolerate more lithium, but lymphoblastoid cells show swelling, indicating potential lithium toxicity risks.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preliminary estimates of the calcium/phosphorus ratio at different cortical bone sites using synchrotron microCT.

Physics in medicine and biology·2006
Same author

An automated approach for analyzing D-periods in collagen fibril images.

IEEE transactions on bio-medical engineering·2005
Same author

Analysis of fibrous proteins from electron microscopy images.

Medical engineering & physics·2005
Same author

High resolution Ca/P maps of bone architecture in 3D synchrotron radiation microtomographic images.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2005
Same author

Development of a reliable and low-cost system for the study of EMF biological effects.

TheScientificWorldJournal·2004
Same author

Biological effects of radiation: role of electron microscopy.

Micron (Oxford, England : 1993)·2001

Area of Science:

  • Nephrology
  • Cell Biology
  • Toxicology

Background:

  • Lithium is a common treatment for mental health conditions.
  • Long-term lithium use is associated with kidney damage.
  • Understanding lithium's cellular effects is crucial for patient safety.

Purpose of the Study:

  • To investigate the morphological effects of lithium chloride on kidney-derived cells.
  • To compare the toxicity of lithium in kidney cells versus non-kidney cells.
  • To determine the concentration-dependent effects and time course of lithium-induced cellular alterations.

Main Methods:

  • Exposure of CV1 (kidney-derived) and HeLa (non-kidney) cells to varying concentrations of lithium chloride.
  • Microscopic observation of cellular morphology, including cell shape, adhesion, and viability.

Related Experiment Videos

  • Assessment of cellular alterations over different treatment durations (up to 4 days).
  • Analysis of morphological changes in a lymphoblastoid cell line.

    • Main Results:

    • Lithium chloride concentrations above 4 mmol were fatal to CV1 cells.
    • HeLa cells demonstrated higher tolerance to lithium chloride compared to CV1 cells.
    • Observed morphological changes in CV1 cells included flattening, detachment, and cell death in a concentration-dependent manner.
    • Lymphoblastoid cells exhibited statistically significant cellular swelling as the primary morphological alteration.

    Conclusions:

    • Lithium chloride exhibits dose-dependent cytotoxicity in kidney-derived cells.
    • Kidney cells are more susceptible to lithium toxicity than non-kidney cells.
    • Cellular swelling in lymphoblastoid cells suggests a potential indicator of lithium exposure and toxicity.