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Characterization of monoclonal antibodies to Yersinia enterocolitica iron-regulated proteins

C Kooi1, P A Sokol

  • 1Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, AB, Canada.

Canadian Journal of Microbiology
|July 1, 1995
PubMed

Insights

Yersinia enterocolitica produces iron-regulated proteins, some on the cell surface, potentially aiding iron acquisition. Antibodies against these proteins showed cross-reactivity but did not prevent infection in mice.

Area of Science:

  • Microbiology
  • Immunology
  • Bacterial Pathogenesis

Background:

  • Yersinia enterocolitica requires iron for growth and virulence.
  • Iron acquisition mechanisms are crucial for bacterial survival in host environments.
  • Understanding iron-regulated proteins is key to developing novel antimicrobial strategies.

Purpose of the Study:

  • To characterize iron-regulated proteins in Yersinia enterocolitica.
  • To investigate the surface exposure and immunological relatedness of these proteins.
  • To assess the potential role of these proteins in iron acquisition and virulence.

Main Methods:

  • Yersinia enterocolitica strains were cultured under varying iron conditions.
  • Proteins were analyzed using SDS-PAGE and Western blotting.
  • Cell surface proteins were identified via iodination.
  • Monoclonal antibodies were generated and characterized for cross-reactivity and functional assays.
  • A mouse infection model was used to evaluate antibody-mediated protection.

Main Results:

  • Several iron-regulated proteins (240, 194, 80, 79, 70, 67 kDa) were identified, with 67 and 70 kDa proteins exposed on the cell surface.
  • Monoclonal antibodies recognized multiple iron-regulated proteins, indicating immunological relatedness and conserved epitopes across serotypes.
  • Antibodies did not inhibit iron uptake or confer protection in a mouse model of Yersinia enterocolitica infection.

Conclusions:

  • Iron-regulated proteins, including surface-exposed ones, are present in Yersinia enterocolitica.
  • These proteins exhibit conserved antigenic sites, suggesting a potential role in iron acquisition.
  • The generated monoclonal antibodies, while useful for characterization, did not target protective epitopes for virulence or iron uptake in this model.

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