Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endogenous lipids in matrix-induced bone morphogenesis

M R Urist1, A J Mikulski

  • 1University of California, Los Angeles School of Medicine, Department of Orthopaedic Surgery 90024-1790, USA.

Clinical Orthopaedics and Related Research
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bone morphodifferentiation and tumorigenesis.

Perspectives in biology and medicine·2015
Same author

A new method of digital skeletal traction.

Medical bulletin. United States. Army. Forces in the European Theater. Office of the Theater Chief Surgeon·2010
Same author

Complete dislocations of the acromiclavicular joint; the nature of the traumatic lesion and effective methods of treatment with an analysis of forty-one cases.

The Journal of bone and joint surgery. American volume·2010
Same author

Calcification and ossification in healing fractures.

Transactions. Conference on Metabolic Aspects of Convalescence·2010
Same author

Injuries to the hip joint traumatic dislocations incurred chiefly in jeep accidents in World War II.

American journal of surgery·2010
Same author

The management of battle-incurred compound fractures in the region of the hip joint.

Military surgeon·2010

Delipidized bone matrix implantation in rats showed limited bone regeneration. Further research is needed to understand lipid roles in bone formation and healing.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Orthopedic Research

Background:

  • Bone matrix is a composite material primarily composed of collagen and hydroxyapatite.
  • Lipids are present in bone matrix and their role in bone regeneration is not fully understood.
  • Complete delipidation of bone matrix is challenging due to the affinity of lipids for collagen.

Purpose of the Study:

  • To investigate the effect of delipidation on the bone inductive capacity of demineralized bone matrix.
  • To explore the role of lipids in the resorption and recalcification of implanted bone matrix.
  • To evaluate the potential of gelatinized bone matrix for bone regeneration.

Main Methods:

  • Demineralized bone matrix was prepared with and without delipidation using chloroform methanol.

Related Experiment Videos

  • Gelatinized bone matrix was delipidated using chloroform methanol, acetone, or both.
  • These matrices were implanted into the muscle tissue of allogeneic rats.

    • Main Results:

    • Demineralized bone matrix without delipidation induced new bone formation and hematopoietic bone marrow.
    • Delipidated bone matrix showed only 20-25% resorption and replacement by new bone, with unresorbed matrix failing to recalcify.
    • Gelatinized bone matrix, delipidated before implantation, exhibited less resorption but showed 12-44% recalcification of the residual matrix.

    Conclusions:

    • Delipidation of bone matrix significantly impairs its osteoinductive potential and ability to form hematopoietic bone marrow.
    • Recalcification of residual matrix does not lead to bone marrow development.
    • Further studies are required to elucidate the role of specific lipids in bone morphogenetic protein delivery and osteogenesis.