Related Experiment Videos
Extracellular adenosine triphosphate. A shock to hemopoietic cells
P J Nijweide1, W E Modderman, C E Hagenaars
1Department of Cell Biology, Faculty of Medicine, Leiden University, The Netherlands.
Clinical Orthopaedics and Related Research
|April 1, 1995
Summary
This study shows that treating bone and cartilage cells with adenosine triphosphate (ATP) and potassium thiocyanate selectively removes hemopoietic cells. This method aids in isolating stromal cells for further research.
Area of Science:
- Cell Biology
- Immunology
- Biochemistry
Background:
- Cellular communication relies on extracellular purinoceptors, including P2-purinoceptors that bind adenosine triphosphate (ATP).
- The P2z-purinoceptor subclass, activated by ATP, increases cell membrane permeability.
- Hemopoietic cells express P2z-purinoceptors, unlike stromal cells, enabling selective cell elimination.
Purpose of the Study:
- To investigate a method for selectively eliminating hemopoietic cells from mixed populations.
- To facilitate the study of stromal cell properties and their interactions with hemopoietic cells.
Main Methods:
- Treatment of isolated bone, bone marrow, and cartilage cell populations.
- Utilizing a combination of adenosine triphosphate (ATP) and potassium thiocyanate.
Main Results:
- Selective obliteration of hemopoietic cells, including macrophages, osteoclasts, and their progenitors.
- Preservation of non-hemopoietic (stromal) cells.
Conclusions:
- A short treatment with ATP and potassium thiocyanate effectively purges hemopoietic cells from skeletal and cartilage tissues.
- This technique provides a valuable tool for isolating and studying stromal cells and their functions.