Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isotope effect studies on the cytochrome P450 enzymes

K R Korzekwa1, J R Gillette, W F Trager

  • 1Laboratory of Molecular Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Drug Metabolism Reviews
|January 1, 1995
PubMed
Summary

Isotope effect experiments reveal key insights into cytochrome P450 enzyme mechanisms. These studies offer unique mechanistic information on oxygen activation and substrate oxidation pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chimeric cDNA expression and site directed mutagenesis studies of cytochrome P450s CYP2A1 and CYP2A2.

The Journal of steroid biochemistry and molecular biology·2012
Same author

Topiramate and phenytoin pharmacokinetics during repetitive monotherapy and combination therapy to epileptic patients.

Epilepsia·2002
Same author

Fluvoxamine-theophylline interaction: gap between in vitro and in vivo inhibition constants toward cytochrome P4501A2.

Clinical pharmacology and therapeutics·2001
Same author

Active site characteristics of CYP4B1 probed with aromatic ligands.

Biochemistry·2001
Same author

Minimal in vivo activation of CYP2C9-mediated flurbiprofen metabolism by dapsone.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2001
Same author

An assessment of the reaction energetics for cytochrome P450-mediated reactions.

Archives of biochemistry and biophysics·2001

Area of Science:

  • Biochemistry
  • Enzymology
  • Chemical Kinetics

Background:

  • Cytochrome P450 enzymes are crucial in drug metabolism and biosynthesis.
  • Understanding their reaction mechanisms is vital for pharmacology and toxicology.
  • Isotope effects are sensitive probes of enzyme reaction pathways.

Purpose of the Study:

  • To highlight the utility of isotope effect experiments in studying cytochrome P450 enzymology.
  • To demonstrate how these experiments elucidate fundamental mechanistic aspects.
  • To showcase the unique insights isotope effects provide into oxygen activation and substrate oxidation.

Main Methods:

  • Utilizing isotope effect studies.
  • Analyzing competition between alternate enzymatic pathways.

Related Experiment Videos

  • Investigating oxygen activation mechanisms.
  • Examining substrate oxidation processes.

    • Main Results:

    • Isotope effect experiments allow for the observation of P450-specific effects.
    • Competition between pathways facilitates the measurement of these effects.
    • Mechanistic details of both oxygen activation and substrate oxidation were obtained.
    • These findings are not easily achievable through other experimental methods.

    Conclusions:

    • Isotope effect studies are indispensable for fundamental cytochrome P450 research.
    • They provide unparalleled mechanistic detail on critical enzymatic steps.
    • This methodology offers unique advantages over alternative approaches for P450 mechanistic investigations.