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Comparison between cDNA clones encoding murine DNA ligase I
1Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Gene
|July 28, 1995
Summary
Researchers isolated a mouse DNA ligase I (LigI) cDNA clone from embryonic stem cells. Six single-amino-acid differences were found compared to a previously described mouse LigI clone.
Area of Science:
- Molecular Biology
- Genetics
Background:
- DNA ligase I (LigI) is crucial for DNA replication and repair.
- Understanding variations in LigI is important for studying its function.
Purpose of the Study:
- To isolate and characterize a mouse DNA ligase I (LigI) cDNA clone.
- To compare the isolated LigI clone with a previously reported mouse LigI cDNA.
Main Methods:
- Isolation of a 3172-nucleotide cDNA clone encoding mouse LigI from an embryonic stem cell cDNA library.
- Sequence comparison between the newly isolated LigI clone and a previously described mouse LigI clone.
Main Results:
- A 3172-nucleotide cDNA clone encoding mouse DNA ligase I (LigI) was successfully isolated.
- Six single-amino-acid alterations were identified between the two mouse LigI cDNA clones.
Conclusions:
- The study provides a new cDNA clone for mouse DNA ligase I.
- Identified sequence variations highlight potential allelic differences or cloning artifacts in mouse LigI research.