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Isolation of a cDNA clone encoding mouse 3-hydroxyacyl CoA dehydrogenase
M Nomura1, Y Takihara, K Shimada
1Department of Medical Genetics, Osaka University, Japan.
Gene
|July 28, 1995
Summary
Researchers identified Rae-38, a mouse cDNA clone with high homology to pig 3-hydroxyacyl CoA dehydrogenase (HCDH). This discovery aids in understanding mammalian HCDH gene regulation.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- 3-hydroxyacyl CoA dehydrogenase (HCDH) is a key enzyme in fatty acid metabolism.
- Understanding the regulation of HCDH gene expression is crucial for metabolic research.
Purpose of the Study:
- To characterize the Rae-38 cDNA clone isolated from mouse embryonal carcinoma F9 cells.
- To investigate the potential of Rae-38 in studying mammalian HCDH gene regulation.
Main Methods:
- Sequencing of the Rae-38 cDNA clone.
- Bioinformatic analysis to compare the deduced RAE-38 protein sequence with known proteins.
Main Results:
- The Rae-38 cDNA clone was successfully sequenced.
- The deduced RAE-38 protein exhibits approximately 86% homology to pig 3-hydroxyacyl CoA dehydrogenase (HCDH).
Conclusions:
- The Rae-38 clone represents a mammalian homolog of HCDH.
- Rae-38 provides a valuable tool for investigating the regulatory mechanisms of HCDH gene expression in mammals.