[Quantitative assessment of blood-aqueous humor barrier permeability using densitometry methods combined with high...

The paper presents an original method of examining blood-aqueous humor permeability. The examinations were performed on grey rabbits that had been intravenously injected 10% fluorescein solution. Subsequently, at definite time intervals from the moment of the administration, fluid of the anterior chamber was taken in which the amount of aqueous fluorescein permeating to aqueous humor was measured. The measurements were performed using the method of high potency thin layer chromatography (HPTLC) in DS type chambers. Examination results were presented as densitometry, and the area of individual points was counted using a computer programme. This method yielded a diagram quantitatively presenting fluorescein permeation from the blood to the aqueous humor.