Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lactoferrin interaction with Actinobacillus actinomycetemcomitans

K R Alugupalli1, S Kalfas, S Edwardsson

  • 1Department of Medical Microbiology, Malmö General Hospital, Lund University, Sweden.

Oral Microbiology and Immunology
|February 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gingival thickness and gingival width in children: a cross-sectional study utilizing ultrasonography.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2024
Same author

Microbial contamination of dental unit water lines - a matter of following instructions.

The Journal of hospital infection·2021
Same author

Low prevalence of multi-resistant bacteria in undergraduate dental students; an observational case-control multi-centre study in Europe.

Journal of oral microbiology·2021
Same author

Streptococcus mutans, Streptococcus sobrinus and Candida albicans in oral samples from caries-free and caries-active children.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2016
Same author

Anthrax Toxin Receptor 1 Is Essential for Arteriogenesis in a Mouse Model of Hindlimb Ischemia.

PloS one·2016
Same author

Milk ribonuclease-enriched lactoferrin induces positive effects on bone turnover markers in postmenopausal women.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2009
Same journal

Influence of mouse prolactin-inducible protein in saliva on the aggregation of oral bacteria.

Oral microbiology and immunology·2009
Same journal

Detection of herpetic viruses in gingival crevicular fluid of patients suffering from periodontal diseases: prevalence and effect of treatment.

Oral microbiology and immunology·2009
Same journal

Characterization of bacterial flora in persistent apical periodontitis lesions.

Oral microbiology and immunology·2009
Same journal

Genetic diversity and toxic activity of Aggregatibacter actinomycetemcomitans isolates.

Oral microbiology and immunology·2009
Same journal

Characterization of L-cysteine desulfhydrase from Prevotella intermedia.

Oral microbiology and immunology·2009
Same journal

Differential virulence and innate immune interactions of Type I and II fimbrial genotypes of Porphyromonas gingivalis.

Oral microbiology and immunology·2009
See all related articles

Lactoferrin specifically binds to outer membrane proteins of Actinobacillus actinomycetemcomitans. This interaction is pH-dependent, reversible, and involves distinct binding sites on the bacterial surface.

Area of Science:

  • Microbiology
  • Immunology
  • Biochemistry

Background:

  • Lactoferrin is an iron-binding glycoprotein with antimicrobial and immunomodulatory functions.
  • Actinobacillus actinomycetemcomitans is a bacterium associated with periodontal disease.

Purpose of the Study:

  • To investigate the specific interaction between lactoferrin and Actinobacillus actinomycetemcomitans.
  • To characterize the binding properties and identify the bacterial targets of lactoferrin.

Main Methods:

  • 125I-labeled protein binding assays.
  • Scatchard analysis.
  • Sodium dodecyl sulfate polyacrylamide-gel electrophoresis (SDS-PAGE) and Western blot analysis.
  • Competitive binding assays.

Related Experiment Videos

Main Results:

  • Lactoferrin binding was pH-dependent, reversible, and occurred optimally within 1 hour.
  • Scatchard analysis revealed two types of binding sites with high and low affinity.
  • Exponential phase bacteria exhibited higher lactoferrin binding compared to stationary phase cells.
  • Lactoferrin-reactive protein bands at 29 kDa and 16.5 kDa were identified in the outer membrane.
  • Heat-inactivated serum, lysed erythrocytes, mucin, and laminin inhibited lactoferrin binding.

Conclusions:

  • Lactoferrin specifically interacts with outer membrane proteins of Actinobacillus actinomycetemcomitans.
  • The binding is influenced by bacterial growth phase and the presence of certain host-derived molecules.
  • These findings contribute to understanding the host-pathogen interactions involving lactoferrin.