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Three-dimensional models of human and other mammalian microsomal P450s constructed from an alignment with P450102
1Molecular Toxicology Group, School of Biological Sciences, University of Surrey, Guildford, UK.
Abstract:
1. A novel modelling alignment for P450s, utilizing NADPH-P450 reductase for electron transfer, is proposed on the basis of analysis of their amino acid sequences. 2. Information used to facilitate the alignment process includes: the recent X-ray crystal structure of P450102 (P450bm3), site-directed mutagenesis experiments, chemical modification of specific residues, and antibody recognition studies. 3. The alignment has been used to construct a number of microsomal P450s of relevance to xenobiotic and endogenous metabolism.