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A unique endoglucanase-encoding gene cloned from the phytopathogenic fungus Macrophomina phaseolina
1Department of Botany & Plant Pathology, Purdue University, West Lafayette, Indiana 47907, USA.
Applied and Environmental Microbiology
|May 1, 1995
Abstract:
The deduced amino acid sequence derived from a Macrophomina phaseolina beta-1,4-endoglucanase-encoding gene revealed 48% identity (over 119 amino acids) with egl1 from the phytopathogen Pseudomonas solanacearum. Its similarity to saprophyte endoglucanases was not significant. Its minimum substrate size, unlike that of any known saprophyte endoglucanase, was cellopentaose. The unique characteristics of M. phaseolina egl1-encoded endoglucanase suggest that it is phytopathogen specific.