Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extensive tissue oxygenation associated with mitochondrial DNA mutations

T Ozawa1, K Sahashi, Y Nakase

  • 1Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.

Biochemical and Biophysical Research Communications
|August 15, 1995
PubMed
Summary

Mitochondrial myopathy patients exhibit tissue oxygenation linked to mitochondrial DNA mutations. This noninvasive method aids in diagnosing and managing this progressive condition, especially in older adults.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Changes in nerve conduction and Pi/PCr ratio during denervation-reinnervation of the gastrocsoleus muscles of rats.

Archives of physical medicine and rehabilitation·1992
Same author

Oxygen radical generation during ischemia-reperfusion in the isolated perfused rat liver monitored by enhanced chemiluminescence.

Circulatory shock·1992
Same author

Phorbol myristate acetate-induced lung injury: involvement of reactive oxygen species.

Scandinavian journal of clinical and laboratory investigation·1992
Same author

The effect of ruthenium red during Ca2+ depletion and repletion in the isolated perfused rat liver.

Research communications in chemical pathology and pharmacology·1992
Same author

Multiparametric evaluation of brain functions in the Mongolian gerbil in vivo.

Journal of basic and clinical physiology and pharmacology·1992
Same author

Dual wavelength spectrophotometry as a diagnostic test of the pulp chamber contents.

Oral surgery, oral medicine, and oral pathology·1992

Area of Science:

  • Biomedical Engineering
  • Molecular Biology
  • Genetics

Background:

  • Mitochondrial myopathies result from mitochondrial DNA (mtDNA) mutations, impacting cellular respiration.
  • Mitochondrial dysfunction leads to impaired oxidative phosphorylation and suppressed oxygen utilization.
  • Progressive mtDNA mutations can exacerbate cellular damage and disease severity.

Observation:

  • Noninvasive tissue oxymetry using near-infrared light was employed.
  • Extensive tissue oxygenation was observed in patients with mitochondrial myopathy.
  • Oxygenation levels were measured in affected tissues.

Findings:

  • Tissue oxygenation extent correlated with the severity of mtDNA mutations.
  • Higher oxygenation indicated more severe defects in oxidative phosphorylation.

Related Experiment Videos

  • Suppressed oxygen utilization was a key characteristic.

    • Implications:

    • This noninvasive technique offers a novel diagnostic approach for mitochondrial myopathies.
    • It can aid in disease management, particularly for elderly patients.
    • Understanding oxygen stress is crucial for tracking disease progression and mutation accumulation.