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Tissue factor antigen levels in various biological fluids

J Fareed1, D D Callas, D Hoppensteadt

  • 1Department of Pathology, Loyola University Medical Center, Maywood, IL 60153, USA.

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|June 1, 1995
PubMed
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Tissue factor (TF) initiates blood clots and is linked to various diseases. This study measured TF levels in patient body fluids using ELISA, finding significant variations across different sample types.

Area of Science:

  • Biochemistry
  • Hematology
  • Immunology

Background:

  • Tissue factor (TF) is a key initiator of thrombogenesis via extrinsic and intrinsic coagulation pathways.
  • Increased TF levels are associated with pathological conditions like cancer, sepsis, inflammation, and cellular necrosis.
  • TF release contributes to post-surgical and post-interventional thrombotic events.

Purpose of the Study:

  • To quantify Tissue Factor (TF) antigen levels in various human biological fluids.
  • To establish reference ranges for TF in normal serum and plasma for comparison.
  • To investigate the presence and concentration of TF in cerebrospinal fluid, peritoneal fluid, pleural effusion, and urine.

Main Methods:

  • Utilized an Enzyme-Linked Immunosorbent Assay (ELISA) employing a murine monoclonal antibody against human TF.

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  • Assayed patient cerebrospinal fluid, peritoneal fluid, pleural effusion, and urine samples.
  • Measured TF antigen levels in normal human serum and plasma as controls.

    • Main Results:

    • Normal human plasma and serum showed TF levels of 165 +/- 139 pg/ml and 165 +/- 110 pg/ml, respectively.
    • Detected TF antigen in cerebrospinal fluid (868 +/- 721 pg/ml), peritoneal fluid (124 +/- 247 pg/ml), pleural effusion (385 +/- 569 pg/ml), synovial fluid (97 +/- 23 pg/ml), seminal plasma (11,485 +/- 875 pg/ml), and urine (86 +/- 57 pg/ml).
    • Demonstrated significant variability in TF concentrations across different bodily fluids.

    Conclusions:

    • The developed ELISA method is effective for assaying TF antigen in diverse biological fluids.
    • TF is present in various human body fluids at quantifiable levels.
    • Further research is warranted to explore the clinical significance of TF levels in these fluids.