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Myelopeptides: bone marrow regulatory mediators
R V Petrov1, A A Mikhailova, L A Fonina
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Bioscience Reports
|February 1, 1995
Summary
Bone marrow cells produce bioregulatory peptides called myelopeptides (MPs). These peptides show immunoregulatory and differentiating activities, with potential therapeutic applications in immune disorders.
Area of Science:
- Biochemistry
- Immunology
- Cell Biology
Background:
- Bone marrow cells from humans and animals produce bioregulatory peptides known as myelopeptides (MPs).
- Myelopeptides possess diverse functional activities, including immunoregulatory, differentiating, and opiate-like effects.
Purpose of the Study:
- To isolate and characterize myelopeptides from porcine bone marrow cell cultures.
- To investigate the immunoregulatory and differentiating effects of myelopeptides.
- To explore the therapeutic potential of myelopeptides in immune disorders and cancer.
Main Methods:
- Reverse phase chromatography was used to isolate and purify myelopeptides.
- Functional activities were assessed through antibody production assays, studies on MRL/lpr mice, and differentiation studies on human HL-60 cells.
- Two individual myelopeptides, MP-1 and MP-2, were isolated and identified.
Main Results:
- Myelopeptides significantly stimulate antibody production (2.5-fold).
- MPs correct immune defects in MRL/lpr mice, prolonging lifespan (2-fold).
- MPs influence the differentiation of normal and leukemic bone marrow and peripheral blood cells, inducing terminal differentiation in HL-60 cells.
Conclusions:
- Myelopeptides exhibit potent immunoregulatory and differentiating activities.
- Myelopeptides hold promise for treating immune deficiencies and autoimmune disorders.
- The immunomodulatory drug Myelopid, based on MPs, is used clinically in Russia for prophylaxis and treatment.