Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoprecipitation isolates multiple TSH receptor forms from human thyroid tissue

R F Grossman1, T Ban, Q Y Duh

  • 1Dept. of Surgery, Mount Zion Medical Center of UCSF, USA.

Thyroid : Official Journal of the American Thyroid Association
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gastropexy predicts lower use of acid-reducing medication after laparoscopic sleeve gastrectomy. A prospective cohort study.

International journal of surgery (London, England)·2020
Same author

Comparison of endoscopic and robotic thyroidectomy.

Annals of surgical oncology·2010
Same author

Incidence and clinical behavior of papillary thyroid carcinoma in renal allograft recipients: a single center experience.

Transplantation proceedings·2008
Same author

Invasion of metastatic human follicular thyroid cancer is inhibited via antagonism of protein kinase C.

Cancer letters·2008
Same author

Cyclooxygenase-2 expression in human thyroid disease.

Journal of endocrinological investigation·2008
Same author

Good blood pressure control on antihypertensives, not only response to spironolactone, predicts improved outcome after adrenalectomy for aldosteronoma.

Surgery·2007
Same journal

Stimulating Thyrotropin Receptor Antibodies Enhance the Expression of Both Thyrotropin Receptors and Insulin-Like Growth Factor 1 Receptors in Fibroblasts.

Thyroid : official journal of the American Thyroid Association·2026
Same journal

Mild Thyroid Eye Disease-The Most Common yet the Least Studied Presentation of Thyroid Eye Disease.

Thyroid : official journal of the American Thyroid Association·2026
Same journal

In Memoriam: Lawrence Crane Wood (1935-2026).

Thyroid : official journal of the American Thyroid Association·2026
Same journal

Trends in Management and Outcomes of Pediatric Hyperthyroidism Over Two Decades.

Thyroid : official journal of the American Thyroid Association·2026
Same journal

Targeted Therapy-Induced Erythrocytosis in Thyroid Cancers: An Underrecognized Safety Signal from a Retrospective Study.

Thyroid : official journal of the American Thyroid Association·2026
Same journal

<i>Retraction Notice:</i> High Iodine Concentration Attenuates <i>RET/PTC3</i> Oncogene Activation in Thyroid Follicular Cells.

Thyroid : official journal of the American Thyroid Association·2026
See all related articles

Researchers purified thyroid-stimulating hormone receptor (TSHR) forms from human thyroid tissue and transfected cells. This breakthrough enables soluble TSHR for future structural and functional studies.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Background:

  • Thyroid-stimulating hormone receptor (TSHR) purification is challenging due to proteolysis and low expression.
  • Previous studies identified multiple TSHR forms (230, 187, 95-100 kDa) in transfected cells, but their presence in human thyroid tissue was unknown.

Purpose of the Study:

  • To develop a method for immunoprecipitating and isolating TSHR forms.
  • To identify and characterize TSHR species in human thyroid tissue and compare them to those in transfected cells.
  • To confirm the single polypeptide structure of the TSHR.

Main Methods:

  • Immunoprecipitation using rabbit polyclonal antiserum against TSHR residues 352-366.
  • Analysis of plasma membranes from transfected COS-7 cells and human thyroid tissue.

Related Experiment Videos

  • Western blot analysis with and without reducing agents.

    • Main Results:

    • Successfully isolated 230 kDa precursor, 187 kDa intermediate, and 95-100 kDa holoreceptor forms of TSHR from both transfected COS-7 cells and human thyroid tissue.
    • Confirmed the single polypeptide nature of TSHR, as the forms remained consistent regardless of reducing agents.
    • This marks the first purification of these TSHR species from transfected cells and their identification in human thyroid tissue.

    Conclusions:

    • Developed a novel immunoprecipitation technique for TSHR purification.
    • Identified multiple TSHR forms in human thyroid tissue, consistent with those found in transfected cells.
    • The purification of soluble TSHR from human tissue paves the way for detailed structural and functional investigations.