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Pasteurella multocida toxin induces IL-6, but not IL-1 alpha or TNF alpha in fibroblasts

S Rosendal1, P L Frandsen, J P Nielsen

  • 1Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.

Canadian Journal of Veterinary Research = Revue Canadienne De Recherche Veterinaire
|April 1, 1995
PubMed

Insights

Pasteurella multocida toxin (PMT) drives atrophic rhinitis in swine by stimulating fibroblast cells. This toxin promotes IL-6 release, offering a new hypothesis for the disease's molecular development.

Area of Science:

  • Veterinary Medicine
  • Molecular Pathogenesis
  • Immunology

Background:

  • Progressive Atrophic Rhinitis (PAR) in swine is primarily caused by Pasteurella multocida toxin (PMT).
  • Previous research indicated PMT's mitogenic effects on fibroblasts.

Purpose of the Study:

  • To confirm the mitogenic effects of PMT on 3T3 fibroblasts.
  • To investigate the cytokine response of fibroblasts stimulated by PMT.
  • To explore the implications for the molecular pathogenesis of PAR.

Main Methods:

  • Cultured 3T3 fibroblasts were stimulated with PMT.
  • Supernatants were analyzed for cytokine release (IL-6, IL-1 alpha, TNF alpha) at 24, 48, and 72 hours post-stimulation.

Main Results:

  • PMT was confirmed to be mitogenic for 3T3 fibroblasts.
  • PMT stimulation induced the release of IL-6 from fibroblasts.
  • IL-1 alpha and TNF alpha were not detected in the supernatants within the observed timeframe.

Conclusions:

  • PMT induces IL-6 release from fibroblasts, contributing to its virulence in swine.
  • The findings support a new hypothesis linking IL-6 to osteoclastic bone resorption in the pathogenesis of PAR.
  • Further research into PMT-induced IL-6 is warranted for understanding and potentially treating swine atrophic rhinitis.

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