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Integrin alpha v subunit is expressed on mesodermal cell surfaces during amphibian gastrulation

D Alfandari1, C A Whittaker, D W DeSimone

  • 1U.R.A. CNRS 1135, Laboratoire de Biologie Moléculaire et Cellulaire du Développement, Université P. et M. Curie, Paris, France.

Developmental Biology
|August 1, 1995
PubMed
Summary

Amphibian gastrulation involves mesodermal cell migration, guided by fibronectin. Researchers identified the alpha v integrin subunit, crucial for this process, and found its expression is developmentally regulated.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Mesodermal cell migration is essential for amphibian gastrulation.
  • Cellular interactions with fibronectin mediate this migration.
  • Alpha v-containing integrin heterodimers are key mediators of fibronectin binding.

Purpose of the Study:

  • To investigate the role of the alpha v integrin subunit in amphibian gastrulation.
  • To clone and characterize the amphibian alpha v integrin subunit.

Main Methods:

  • Homology PCR was used to clone the Pleurodeles homologue of the integrin alpha v subunit.
  • Amino acid sequence identity was compared to human and chick homologues.
  • mRNA expression and protein localization were analyzed during oogenesis and embryonic development.

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Main Results:

  • The deduced amino acid sequence of the amphibian alpha v subunit shows high identity with human (73%) and chick (74%) homologues.
  • Alpha v mRNA (4.8-kb) is expressed during oogenesis and throughout development.
  • While mRNA and protein are widely distributed, cell surface expression is spatially regulated, appearing on oocytes and later on mesodermal cells during gastrulation.

Conclusions:

  • Alpha v integrin expression is developmentally regulated via a post-translational mechanism.
  • This regulation correlates with the initiation of mesodermal cell migration during gastrulation.
  • The alpha v subunit plays a critical role in mediating cell migration during amphibian development.