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Expression and purification of Clostridium perfringens beta-toxin glutathione S-transferase fusion protein
V Steinthorsdottir1, V Fridriksdottir, E Gunnarsson
1Institute for Experimental Pathology, University of Iceland, Reykjavík.
FEMS Microbiology Letters
|August 1, 1995
Abstract:
The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid difference between the two proteins. The purified beta-toxin fusion protein is not toxic in mice, but rabbit antiserum raised against it neutralises the toxic effect of C. perfringens type C culture filtrate in mice.