B- and T-cell markers on human lymphoblasts after stimulation with mitogen or antigens

Receptors for sheep erythrocytes and for the third component of complement were demonstrated on human lymphoblasts after stimulation with various antigens and mitogens. With these markers B- and T-cell stimulation could be differentiated. Phytohaemagglutinin (PHA) and purified protein derivative (PPD) proved to activate predominantly T cells, whereas foetal calf serum (FCS) was shown to be a B-cell stimulator. Candida albicans and allogeneic cells, on the other hand, stimulated both B and T cells.