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T cell repertoire in systemic sclerosis

V V Yurovsky1, B White

  • 1Department of Medicine, University of Maryland, Baltimore 21201, USA.

International Reviews of Immunology
|January 1, 1995
PubMed
Summary
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Systemic sclerosis patients show increased T cells, with restricted gene diversity in V delta 1, V alpha, and V beta T cell receptors. This suggests antigen-driven T cell expansion may drive disease pathogenesis.

Area of Science:

  • Immunology
  • Molecular Biology
  • Rheumatology

Background:

  • Systemic sclerosis (SSc) is characterized by an increase in specific T cell subsets, including V delta 1+ gamma delta T cells and CD8+ alpha beta T cells.
  • The role of T cell receptor (TCR) repertoire diversity in SSc pathogenesis is not fully understood.

Purpose of the Study:

  • To investigate the diversity of T cell receptor (TCR) gene repertoires in patients with systemic sclerosis.
  • To determine if restricted TCR diversity is associated with specific T cell subsets in SSc patients.

Main Methods:

  • Utilized reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify rearranged TCR transcripts.
  • Analyzed the relative expression of V alpha and V beta genes in blood and bronchoalveolar lavage (BAL) fluid.

Related Experiment Videos

  • Examined junctional region diversity of TCR V delta 1, V alpha, and V beta transcripts, including sequence analysis and length distribution.

    • Main Results:

    • Observed limited V delta 1-C delta junctional region lengths in SSc patients compared to controls.
    • Detected restricted diversity in junctional region lengths for several V alpha and V beta gene families, particularly in bronchoalveolar CD8+ T cells.
    • Confirmed limited V delta 1-C delta junctional diversity through sequence analysis after subcloning.

    Conclusions:

    • The findings suggest an oligoclonal expansion of alpha beta and gamma delta T cells in systemic sclerosis.
    • This expansion appears to be antigen-driven, indicating a potential role in the pathogenesis of SSc.
    • TCR repertoire analysis provides insights into the immune mechanisms underlying systemic sclerosis.