Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HLA-DR polymorphism affects the interaction with CD4

S Fleury1, J Thibodeau, G Croteau

  • 1Laboratoire d'Immunologie, Institut de Recherches Clinques de Montréal, Québec, Canada.

The Journal of Experimental Medicine
|September 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards heat health plans in sub-Saharan Africa: case study - Nigeria.

Perspectives in public health·2025
Same author

Novel paradigm enables accurate monthly gestational screening to prevent congenital toxoplasmosis and more.

medRxiv : the preprint server for health sciences·2023
Same author

Relevance and Feasibility of a Personalized Dietary Intervention in Older People with Meals-On-Wheels: A Randomized Controlled Pilot Trial.

JAR life·2023
Same author

High total cholesterol and triglycerides levels increase arginases metabolism, impairing nitric oxide signaling and worsening fetoplacental endothelial dysfunction in gestational diabetes mellitus pregnancies.

Biochimica et biophysica acta. Molecular basis of disease·2021
Same author

Exposure to contaminants and nutritional intakes in a French vegetarian population.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2017
Same author

Introduction.

Immunologic research·2016
Same journal

Retraction: In vivo NCL targeting affects breast cancer aggressiveness through miRNA regulation.

The Journal of experimental medicine·2026
Same journal

Intravesical mesothelin-based CAR T cells targeting MUC16 effectively control bladder cancer in preclinical models.

The Journal of experimental medicine·2026
Same journal

Flawed translation triggers oncogenic B-T cell communication.

The Journal of experimental medicine·2026
Same journal

Correction: LCK'ed in: Inborn errors of immunity in LCK reveal how TCR signaling is calibrated.

The Journal of experimental medicine·2026
Same journal

Mechanobiology of inflammation: Pulling the strings of innate immunity.

The Journal of experimental medicine·2026
Same journal

Bile acid retention in efferocytic macrophages shapes their inflammatory status during cholangitis.

The Journal of experimental medicine·2026
See all related articles

Allelic polymorphism in Major Histocompatibility Complex (MHC) class II molecules influences their interaction with CD4. Specific residues between positions beta 180 and 189 significantly impact this crucial T cell recognition.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Major histocompatibility complex (MHC) class II molecules present peptides to CD4+ T cells.
  • CD4 interaction with MHC class II enhances T cell responses, involving the p56lck kinase.
  • The polymorphic nature of MHC class II suggests allelic variations may affect CD4 binding.

Purpose of the Study:

  • To investigate the impact of allelic polymorphism in MHC class II molecules on their interaction with CD4.
  • To identify specific regions or residues responsible for differential CD4 binding.
  • To understand how MHC class II diversity influences T cell activation.

Main Methods:

  • Utilized mouse DAP-3-transfected cells expressing various HLA-DR isotypes and alleles.
  • Performed functional assays to measure CD4 interaction with different MHC class II molecules.

Related Experiment Videos

  • Constructed chimeric MHC class II molecules (DR4/DR1 with DRw53) to map critical polymorphic residues.

    • Main Results:

    • A hierarchy of CD4 interaction was observed among different MHC class II molecules.
    • The study minimized the role of peptide-binding groove polymorphisms in CD4 interaction.
    • Polymorphic residues between beta positions 180 and 189 were identified as critical for modulating CD4 interaction strength.

    Conclusions:

    • Allelic variations in MHC class II molecules significantly influence CD4 binding affinity.
    • Specific polymorphic residues within a defined region dictate the efficiency of CD4-MHC class II interaction.
    • These findings provide insights into the molecular basis of T cell recognition and immune response modulation.