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Mechanisms of regulatory volume decrease in collecting duct cells

W J Fu1, M Kuwahara, F Marumo

  • 1Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.

The Japanese Journal of Physiology
|January 1, 1995
PubMed
Summary
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Rabbit kidney cells regulate volume by releasing potassium and chloride ions. Intracellular calcium also plays a role in this regulatory volume decrease (RVD) mechanism.

Area of Science:

  • Nephrology
  • Cell Physiology
  • Renal Physiology

Background:

  • Cell volume regulation is crucial for kidney function.
  • The outer medullary collecting duct (OMCDi) plays a role in renal concentrating ability.
  • Osmotic stress triggers cellular responses, including regulatory volume decrease (RVD).

Purpose of the Study:

  • To investigate the ionic mechanisms of regulatory volume decrease (RVD) in rabbit OMCDi cells.
  • To elucidate the role of intracellular calcium in RVD.
  • To identify the specific ion transporters involved in cell volume regulation.

Main Methods:

  • Dissection and in vitro perfusion of rabbit kidney OMCDi segments.
  • Real-time monitoring of cell cross-sectional area using image processing.

Related Experiment Videos

  • Pharmacological manipulation of ion concentrations (K+, Cl-, Ba2+) and intracellular calcium (BAPTA-AM).

    • Main Results:

    • OMCDi cells exhibited RVD following osmotic swelling.
    • Basolateral K+ and Cl- exit significantly inhibited RVD.
    • Intracellular Ca2+ rise accompanied RVD, and its chelation impaired the response.
    • Basolateral Ba2+ and anthracene-9-CO2H inhibited RVD.

    Conclusions:

    • Basolateral K+ and Cl- conductive pathways are key mediators of RVD in rabbit OMCDi cells.
    • Intracellular calcium signaling is involved in the RVD process.
    • These findings enhance understanding of renal cell volume homeostasis.