Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identification of type C viruses by electron microscopy

R C Lo, A F Howatson

    Cancer Research
    |April 1, 1978
    PubMed
    Summary

    Electron microscopy reveals a distinct "double-ring" core structure in type C viruses. Detergent treatment significantly enhances the detection of these viral particles, even among other particulates.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Clinical relevance of cranial nerve injury following carotid endarterectomy.

    European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2013
    Same author

    Recovery and rehabilitation after stroke.

    Canadian family physician Medecin de famille canadien·2011
    Same author

    MRSA - again.

    British journal of plastic surgery·2001
    Same author

    Transient global amnesia associated with acute heparin-induced thrombocytopenia.

    The American journal of medicine·1994
    Same author

    Microfilaments associated with Paget's disease of bone: comparison with nucleocapsids of measles virus and respiratory syncytial virus.

    Intervirology·1982
    Same author

    Colony formation by normal and malignant human B-lymphocytes.

    British journal of cancer·1980

    Area of Science:

    • Virology
    • Microscopy techniques

    Background:

    • Type C viruses possess a characteristic "double-ring" core structure.
    • This feature is observable via electron microscopy using negative staining or thin sectioning.
    • Recognizing viral particles in impure samples can be challenging.

    Purpose of the Study:

    • To characterize the "double-ring" core of type C virus particles.
    • To assess methods for enhancing the detectability of these viral particles.
    • To determine the limit of detection for type C virus in complex mixtures.

    Main Methods:

    • Electron microscopy (negative staining and thin sectioning).
    • Treatment with non-ionic detergent prior to fixation and staining.
    • Assessment of particle detectability in the presence of nonviral contaminants.

    Main Results:

    • A distinct "double-ring" appearance was identified in type C virus cores.
    • Non-ionic detergent treatment significantly improved the visibility of double-ring particles.
    • The study established the detectability limit of type C virus in impure preparations.

    Conclusions:

    • The "double-ring" core is a reliable marker for identifying type C viruses.
    • Detergent treatment is a valuable method for enhancing viral particle detection in electron microscopy.
    • This technique improves the ability to identify type C virus in complex biological samples.

    Related Experiment Videos