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Multiple domains mediate heme control of the yeast activator HAP1
1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Molecular & General Genetics : MGG
|July 28, 1995
Abstract:
The activity of the yeast activator HAP1 in vivo requires heme. A heme responsive domain of HAP1 was identified previously. It is adjacent to the DNA-binding domain and appears to block DNA binding in the absence of heme. Here we describe a novel genetic selection which yielded mutants of HAP1 that are independent of heme when assayed for activation. These mutants define a second region of HAP1, close to the activation domain, which also controls its response to heme.