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Updated: Aug 6, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
Published on: June 21, 2016
Trans-activation and DNA-binding properties of the transcription factor, Sox-18
B M Hosking1, G E Muscat, P A Koopman
1Centre for Molecular and Cellular Biology, University of Queensland, St Lucia, Australia.
Abstract:
Sox-18 is a member of the Sox multi-gene family (Sry-related HMG-box gene). We have bacterially expressed this 378 amino acid protein and demonstrated sequence-specific binding to the Sox DNA-binding motif AACAAAG. A distinct 95 amino acid activation domain was mapped in Sox-18 using GAL4-Sox-18 fusions (amino acids 160-225). Furthermore, Sox-18 was capable of trans-activating gene expression through the AACAAA motif. Our results suggest that Sox-18 functions as a classical trans-activator of gene expression.
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