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Partial characterization of an immunoenhancing factor from allogeneic human lymphocyte cell lines
Immunology
|January 1, 1976
Summary
Researchers discovered a human enhancing factor (HEF) secreted by mixed lymphocyte cultures. This factor non-specifically boosts the immune response of mouse spleen cells in vitro.
Area of Science:
- Immunology
- Cell Biology
Background:
- Histoincompatible human lymphocyte cell lines release soluble factors.
- These factors can modulate immune responses.
Purpose of the Study:
- To characterize a soluble factor that enhances in vitro immune responses.
- To determine the properties and molecular weight of this human enhancing factor (HEF).
Main Methods:
- Mixing of histoincompatible human lymphocyte cell lines.
- Assaying immune response enhancement in normal mouse spleen cells against sheep erythrocytes.
- Purification using ammonium sulphate fractionation, Sephadex gel filtration, DEAE-cellulose chromatography, and SDS-PAGE.
Main Results:
- A soluble factor, HEF, was secreted within 24 hours.
- HEF activity requires addition on day 2 of a 5-day culture.
- HEF is resistant to DNase, RNase, and 56°C heat, but inactivated by protease and 80°C heat.
- Purified HEF has a molecular weight of approximately 38,000 Daltons.
Conclusions:
- A novel human enhancing factor (HEF) with immunomodulatory properties was identified.
- HEF exhibits specific stability characteristics and a defined molecular weight.
- This factor holds potential for understanding and manipulating immune responses.