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Participation of monocytes and macrophages in canine glomerular disease
M Vilafranca1, L Ferrer, P Wohlsein
1Department of Animal Pathology, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Spain.
Abstract:
The presence of monocytes/macrophages (MPs) in renal glomeruli was investigated in 86 dogs with different types of glomerulopathies. The identification of MPs in tissue sections was based on cytological criteria and the immunohistochemical demonstration of lysozyme. The highest numbers of MPs in glomeruli were observed in focal and diffuse mesangial proliferative glomerulonephritis (MesPGN), diffuse endocapillary proliferative glomerulonephritis (DEPGN), and diffuse mesangiocapillary glomerulonephritis (DMCGN). In cases of minor glomerular abnormalities (MGA), focal and segmental hyalinosis and sclerosis (FGS), diffuse membranous glomerulonephritis (DMemGN), and diffuse sclerosing glomerulonephritis (DSGN) the presence of glomerular MPs was low. Particularly in MesPGN, the number of MPs was correlated with glomerular hypercellularity and, additionally in DMCGN, with the degree of proteinuria. The results of this study suggest that MPs may be involved in functional and morphological alterations in different types of canine glomerulopathies.
Insights
Monocytes/macrophages (MPs) in dog kidneys were studied in various glomerulopathies. Higher MP numbers were found in proliferative and mesangiocapillary types, suggesting their role in kidney disease progression.
Area of Science:
- Veterinary Pathology
- Immunohistochemistry
- Canine Nephrology
Background:
- Glomerulopathies are common kidney diseases in dogs.
- Monocytes/macrophages (MPs) are key immune cells implicated in renal pathology.
- Understanding MP roles in canine glomerulonephritis is crucial for diagnosis and treatment.
Purpose of the Study:
- To investigate the presence and distribution of MPs in canine renal glomeruli across different glomerulopathy types.
- To correlate MP numbers with specific glomerulonephritis classifications and clinical parameters like proteinuria.
Main Methods:
- Analysis of 86 canine kidney biopsy samples with various glomerulopathies.
- Identification of MPs using cytological criteria and lysozyme immunohistochemistry.
- Quantification of glomerular MPs and correlation with disease type and proteinuria.
Main Results:
- Elevated glomerular MP numbers were observed in focal/diffuse mesangial proliferative glomerulonephritis (MesPGN), diffuse endocapillary proliferative glomerulonephritis (DEPGN), and diffuse mesangiocapillary glomerulonephritis (DMCGN).
- Low MP presence was noted in minor glomerular abnormalities (MGA), focal/segmental hyalinosis and sclerosis (FGS), diffuse membranous glomerulonephritis (DMemGN), and diffuse sclerosing glomerulonephritis (DSGN).
- MP counts correlated with glomerular hypercellularity in MesPGN and proteinuria in DMCGN.
Conclusions:
- Monocytes/macrophages are significantly present in glomeruli of dogs with specific proliferative and mesangiocapillary glomerulonephritides.
- These findings suggest MPs play a role in the functional and morphological changes associated with canine glomerulopathies.
- Further research into MP functions could offer therapeutic targets for canine kidney disease.