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Cleavage of DNA by N-phosphoryl histidine
1Bio-organic Phosphorus Chemistry Laboratory, School of Life Sciences and Engineering, Tsinghua University, Beijing, China.
Biochemical and Biophysical Research Communications
|August 24, 1995
Abstract:
DNA cleavage by N-(O,O-diisopropyl)phosphoryl histidine(DIPP-His) is investigated by a submarine agarose electrophoresis technique. It was found that DIPP-His could cleave supercoiled DNAs and lambda DNA without the participation of divalent metal ions, while the histidine itself and N-(O,O-diisopropyl)phosphoryl alanine had no such function. Supercoiled pUC8 and pBR322 DNAs are cleaved to linear DNA and eventually fragments; lambda DNA is cleaved directly to fragments. Two mechanistic pathways are proposed for the cleavage reaction.