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Programmed cell death: early changes in metamorphosing cells

R A Lockshin1, Z Zakeri

  • 1Department of Biological Sciences, St. John's University, Jamaica, NY 11439, USA.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|November 1, 1994
PubMed
Summary
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Programmed cell death in Manduca sexta involves early lysosomal activity and delayed nuclear collapse. Protein synthesis declines sharply, but the exact cause remains unclear.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Insect Physiology

Background:

  • Programmed cell death (PCD) is crucial for development and tissue homeostasis.
  • Type II PCD, characterized by lysosomal activity, is observed in various biological contexts.
  • Understanding the molecular mechanisms of PCD in insects provides insights into conserved cellular processes.

Purpose of the Study:

  • To investigate the morphological and molecular events during programmed cell death in Manduca sexta intersegmental muscles and labial glands.
  • To identify key features and potential triggers of type II PCD in these tissues.

Main Methods:

  • Morphological analysis of cellular changes during degeneration.
  • Biochemical assays to assess protein synthesis and energy resources.

Related Experiment Videos

  • Molecular analysis of mRNA levels.

    • Main Results:

    • Programmed cell death in Manduca sexta exhibits features of type II PCD, with prominent early lysosomal activity and late nuclear collapse.
    • A significant decrease in overall protein synthesis occurs, while some mRNAs persist or are upregulated.
    • Energy resources and second messenger levels do not fully explain the observed tissue failure.

    Conclusions:

    • Lysosomal degradation is a key early event in Manduca sexta programmed cell death.
    • The precipitous drop in protein synthesis is not fully explained by current data, suggesting further investigation is needed.
    • Potential DNA instability may contribute to tissue failure under load.