Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein analysis on a genomic scale

P Jungblut1, B Wittmann-Liebold

  • 1Wittmann Institute of Technology and Analysis of Biomolecules, Teltow, Germany.

Journal of Biotechnology
|July 31, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Purification and characterization of ten new rice NaCl-soluble proteins: identification of four protein-synthesis inhibitors and two immunoglobulin-binding proteins.

Planta·2013
Same author

Purification and characterization of ribosomal proteins from the 30 S subunit of the extreme halophile Halobacterium marismortui.

FEBS letters·2012
Same author

Microsequencing of Peptides and Proteins with 4-N,N-Dimethylaminoazobenzene-4'-isothiocyanate.

Methods in molecular biology (Clifton, N.J.)·2010
Same author

Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiella sp. ASR1.

Applied microbiology and biotechnology·2004
Same author

Apoptosis-induced cleavage of keratin 15 and keratin 17 in a human breast epithelial cell line.

Cell death and differentiation·2001
Same author

Heart-specific splice-variant of a human mitochondrial ribosomal protein (mRNA processing; tissue specific splicing).

Gene·2001

Traditional protein analysis focused on single proteins. Two-dimensional electrophoresis (2-DE) allows visualization and analysis of complex protein variations, enabling genomic-scale investigations and gene identification.

Area of Science:

  • Proteomics
  • Molecular Biology
  • Biochemistry

Background:

  • Classical protein analysis methods often use a reductionist approach, focusing on individual proteins isolated from complex biological mixtures.
  • The intricate nature of protein composition in biological systems necessitates advanced visualization techniques.

Purpose of the Study:

  • To introduce two-dimensional electrophoresis (2-DE) as a method to visualize and analyze complex protein variations.
  • To demonstrate how 2-DE can be combined with other techniques for protein identification and gene discovery.
  • To highlight the capability of 2-DE for analyzing complex protein changes on a genomic scale.

Main Methods:

  • Two-dimensional electrophoresis (2-DE) for visualizing protein complexity.
  • Subtractive 2-DE analyses for detecting protein variations.

Related Experiment Videos

  • Integration of 2-DE with microsequencing, amino acid analysis, and mass spectrometry for protein identification.
  • Gene identification based on obtained sequence information.

    • Main Results:

    • 2-DE provides a comprehensive view of protein composition, overcoming limitations of reductionist approaches.
    • Subtractive 2-DE enables the detection of subtle protein variations between different biological states.
    • Combined techniques allow for the identification of specific protein spots and subsequent gene discovery.
    • 2-DE facilitates the analysis of complex, multi-protein changes across the genome.

    Conclusions:

    • Two-dimensional electrophoresis (2-DE) offers a powerful tool for visualizing and analyzing the complexity of proteomes.
    • This technique, when coupled with identification methods, enables detailed protein and gene-level investigations.
    • 2-DE supports the study of both single protein alterations and large-scale genomic changes in protein expression.