Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Highly efficient eukaryotic gene expression vectors for peptide secretion

T H Chu1, I Martinez, P Olson

  • 1University of Medicine and Dentistry of New Jersey, Piscataway, USA.

Peptide Research
|March 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visual evoked potentials and development on children with congenital cataract with surgery after 4 months of age.

Archivos de la Sociedad Espanola de Oftalmologia·2025
Same author

Inhibition of calreticulin by protein nanoparticles carrying antisense oligonucleotides reduce gene expression and infection of <i>Trypanosoma cruzi</i> trypomastigotes on mammalian cells.

Nanomedicine (London, England)·2025
Same author

Production of poly-3-hydroxybutyrate (PHB) nanoparticles using grape residues as the sole carbon source.

International journal of biological macromolecules·2024
Same author

Clinical findings, surgical treatment and outcome in dogs with parotid duct ectasia: 14 cases (2010-2023).

The Journal of small animal practice·2023
Same author

[Translated article] Tumor ablation and vertebral augmentation in the treatment of vertebral metastases: A multicenter study.

Revista espanola de cirugia ortopedica y traumatologia·2023
Same author

Major Fusarium species and mycotoxins associated with freshly harvested maize grain in Uruguay.

Mycotoxin research·2023
Same journal

Solution structure of vasoactive intestinal polypeptide (11-28)-NH2, a fragment with analgesic properties.

Peptide research·1996
Same journal

NMR study of five N-terminal peptide fragments of the vasoactive intestinal peptide: crucial role of aromatic residues.

Peptide research·1996
Same journal

Crystallographic characterization of tryptophan-containing peptide 3(10)-helices.

Peptide research·1996
Same journal

Assignment of the disulfide bonds in napin, a seed storage protein from Brassica napus, using matrix-assisted laser desorption ionization mass spectrometry.

Peptide research·1996
Same journal

Effective use of free thiols as scavengers for HF cocktails to deprotect bromo- and chloroacetylated synthetic peptides.

Peptide research·1996
Same journal

Large-pore polydimethylacrylamide resin for solid-phase peptide synthesis: applications in Fmoc chemistry.

Peptide research·1996
See all related articles

New universal eukaryotic gene expression vectors facilitate the secretion and cell membrane incorporation of peptides. These modified vectors enable efficient protein transport and simplify the construction of chimeric transmembrane or secretion proteins.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Cell Biology

Background:

  • Eukaryotic gene expression vectors are essential tools in molecular biology.
  • Previous universal vectors offered a foundation for gene expression.
  • Need for vectors enabling protein secretion or membrane integration.

Purpose of the Study:

  • To modify existing universal eukaryotic gene expression vectors for enhanced protein secretion and membrane incorporation.
  • To develop a streamlined protocol for cloning peptide-coding regions for expression and secretion.
  • To assess the efficiency of protein transport using the modified vectors.

Main Methods:

  • Construction of modified universal eukaryotic gene expression vectors.
  • Insertion of stop codons and an endoplasmic reticulum (ER) signal peptide sequence.

Related Experiment Videos

  • Utilizing PCR products for cloning peptide-coding regions.
  • Evaluating the transport efficiency of chimeric transmembrane proteins.

    • Main Results:

    • Modified vectors successfully enabled expression of secreted and membrane-bound proteins.
    • The ER signal peptide facilitated efficient protein transport through the endoplasmic reticulum.
    • Transport efficiency of chimeric proteins with the ER leader was comparable to native proteins.
    • A fast and easy cloning protocol for peptide-coding regions was established.

    Conclusions:

    • The developed universal vectors are highly efficient for expressing secreted and transmembrane proteins.
    • The modifications allow for straightforward construction of chimeric proteins for various applications.
    • These vectors simplify the study of protein localization and function in eukaryotic cells.