Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

pBINPLUS: an improved plant transformation vector based on pBIN19

F A van Engelen1, J W Molthoff, A J Conner

  • 1Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Molecular Biology, Wageningen, The Netherlands.

Transgenic Research
|July 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Are we safe? Exploring psychological factors affecting radiation risk perception of dwellers in old uranium mining quarters.

Annals of the ICRP·2026
Same author

Relationship between hair cortisol and plasma heat shock proteins in response to heat stress in tolerant and sensitive cattle breeds.

Scientific reports·2026
Same author

Addressing Critical Fungal Pathogens Under a One Health Perspective: Key Insights from the Portuguese Association of Medical Mycology.

Mycopathologia·2025
Same author

High dietary total energy intake and energy density are associated with girls' breast density during puberty.

European journal of nutrition·2025
Same author

Serum and saliva immunoglobulin (immunoglobulin G, immunoglobulin A, and immunoglobulin M) dynamics in newborn calves and their association with health status during the first week of life: An exploratory study.

Journal of dairy science·2025
Same author

Central nervous system vascular complications associated with the acute form of steroid-responsive meningitis-arteritis.

Veterinary journal (London, England : 1997)·2025
Same journal

Correction: Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases.

Transgenic research·2026
Same journal

Effects of CRISPR technology on agricultural sustainability: global applications and turkish perspective.

Transgenic research·2026
Same journal

Mammalian growth factors enhance regeneration in transgenic tomato lines.

Transgenic research·2026
Same journal

Transgenic expression of human signal regulatory protein alpha (SIRPα) in BALB/c Rag2<sup>null</sup>/Jak3<sup>null</sup> immunodeficient mice enhances human lymphoma xenografts by reducing phagocytosis.

Transgenic research·2026
Same journal

Evaluation of different mutagens in tuberose (Polianthes tuberosa) cut flower.

Transgenic research·2026
Same journal

Suppressor activity against cosuppression conferred by the petunia vein clearing virus genome.

Transgenic research·2026
See all related articles

Researchers developed pBINPLUS, an improved plant transformation vector based on pBIN19. This new vector facilitates easier cloning and analysis of plant genome insertions, enhancing genetic research.

Area of Science:

  • Molecular Biology
  • Plant Biotechnology

Background:

  • The pBIN19 vector is widely used for plant transformation.
  • Existing vectors may present challenges in cloning and analysis of T-DNA insertions.

Purpose of the Study:

  • To construct and characterize a novel plant transformation vector, pBINPLUS.
  • To improve upon the functionalities of the pBIN19 vector for enhanced plant genetic engineering.

Main Methods:

  • Construction of the pBINPLUS vector, a derivative of pBIN19.
  • Modification involved strategic placement of the selectable marker gene and incorporation of rare restriction sites.

Main Results:

  • The pBINPLUS vector features a selectable marker gene at the left T-DNA border.

Related Experiment Videos

  • It exhibits a higher copy number in E. coli compared to pBIN19.
  • Two rare restriction sites are integrated around the multiple cloning site for streamlined cloning.

    • Conclusions:

    • pBINPLUS offers significant advantages over pBIN19 for plant transformation.
    • The vector design facilitates more efficient cloning and analysis of T-DNA insertions in plant genomes.
    • This advancement aids in plant genetic research and biotechnology applications.