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[Technics for isolation of ATP from microorganisms]

S I Aksenov, L N Sosunova

    Mikrobiologiia
    |November 1, 1975
    PubMed
    Summary

    A novel dehydration and boiling water method efficiently isolates adenosine triphosphate (ATP) from both eukaryotic and prokaryotic microorganisms. This technique enhances cell membrane permeability, yielding more ATP for immediate experimental measurement.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Cell Biology

    Background:

    • Accurate quantification of adenosine triphosphate (ATP) is crucial for understanding cellular energy metabolism.
    • Existing methods for ATP isolation from microorganisms often face challenges with yield and efficiency.
    • Cell membrane integrity can limit the release of intracellular ATP during extraction.

    Purpose of the Study:

    • To develop an improved technique for the isolation of ATP from diverse microbial sources.
    • To enhance the yield of ATP extraction by increasing microbial cell membrane permeability.
    • To provide a method for the real-time measurement of ATP levels in microbial samples.

    Main Methods:

    • A novel technique involving dehydration of microbial biomass.
    • Treatment of dehydrated cells with boiling water to increase cell membrane permeability.
    • Isolation and quantification of released ATP from the treated microbial samples.

    Main Results:

    • The proposed technique significantly increases the yield of ATP from both eukaryotic and prokaryotic microorganisms compared to conventional methods.
    • Dehydration followed by boiling water treatment effectively enhances cell membrane permeability for low molecular weight substances like ATP.
    • The method allows for the direct measurement of ATP at the time of the experiment, preserving its physiological state.

    Conclusions:

    • The developed method offers a superior approach for ATP isolation from microorganisms, enhancing extraction efficiency.
    • Increased cell membrane permeability during dehydration is key to the improved ATP yield.
    • This technique facilitates more accurate and timely assessment of microbial energy status.

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