[Primary-explant method of culturing human arterial smooth muscle cells]

The primary culture of human arterial smooth cells (SMC) was performed successfully using the primary-explant method. The cells formed "hills and valleys" characteristic of SMC; the myofilament bundles were observed in the cytoplasm of the cells under an electron microscope and the cells were stained red with Ma's method. The growing character of 35th passage SMC kept unchanged. In addition, some problems concerning the culture of human arterial smooth muscle cells are discussed.