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Plasmin in pericellular proteolysis and cellular invasion
M D Kramer1, J Reinartz, G Brunner
1Institute for Immunology and Serology, University of Heidelberg, Germany.
Invasion & Metastasis
|January 1, 1994
Summary
Cellular plasmin generation impacts cell adhesion to fibrin clots and promotes melanoma cell invasion. This process is crucial for understanding tumor invasion and inflammation dynamics.
Area of Science:
- Biochemistry
- Cell Biology
- Oncology
Background:
- Tumor invasion and inflammation involve fibrinogen extravasation and clot formation.
- Fibrin clots serve as a matrix for cell migration during these processes.
- The specific factors governing cell-fibrin interactions remain largely unknown.
Purpose of the Study:
- To investigate the role of cell-surface plasminogen activation in keratinocyte adhesion to fibrin.
- To examine the impact of cell-surface plasmin generation on melanoma cell invasion into fibrin gels.
- To elucidate the mechanisms of cell-fibrin interactions in invasive conditions.
Main Methods:
- In vitro experiments using keratinocytes and melanoma cells.
- Assessing cell adhesion to fibrin matrices.
- Measuring cell invasion through three-dimensional fibrin gels.
- Analyzing the effects of cell-surface plasmin generation.
Main Results:
- Plasmin generation at the cell surface destabilizes keratinocyte adhesion to fibrin by cleaving fibrin.
- Cell-surface plasmin promotes melanoma cell invasion into fibrin matrices.
- Evidence suggests plasmin's role in pericellular proteolysis and cell-matrix interactions.
Conclusions:
- Cell-surface plasmin generation is a key factor in pericellular proteolysis.
- This process dynamically influences cell interactions with fibrin-rich matrices.
- Understanding plasmin's role is vital for conditions involving tumor invasion and inflammation.