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Related Experiment Videos

Microsatellite markers in white-tailed deer

J A DeWoody1, R L Honeycutt, L C Skow

  • 1Department of Veterinary Anatomy and Public Health, Texas A&M University, College Station 77843, USA.

The Journal of Heredity
|July 1, 1995
PubMed
Summary
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Researchers developed microsatellite markers for white-tailed deer DNA, aiding genetic studies in artiodactyls. These markers provide insights into deer population genetics and evolutionary relationships.

Area of Science:

  • Genomics
  • Molecular Biology
  • Population Genetics

Background:

  • Microsatellite repeats are valuable genetic markers for population studies.
  • Developing species-specific markers is crucial for understanding genetic diversity.

Purpose of the Study:

  • To construct a genomic library for white-tailed deer DNA.
  • To isolate and characterize d(AC)n microsatellite repeats.
  • To develop polymorphic markers for genetic analysis in white-tailed deer and related species.

Main Methods:

  • Construction of a white-tailed deer genomic DNA library.
  • Polymerase Chain Reaction (PCR) amplification using primers flanking microsatellite repeats.
  • Analysis of allelic frequencies, Polymorphic Information Content (PIC), and heterozygosity.

Related Experiment Videos

  • Somatic cell hybrid mapping to assign marker homologs to bovine chromosomes.
  • Main Results:

    • Five polymorphic microsatellite markers were identified and characterized.
    • Allelic frequencies, PIC, and heterozygosity values were determined for 41 white-tailed deer.
    • Two markers were successfully mapped to bovine chromosomes 4 and 23 using hybrid cell lines.

    Conclusions:

    • The developed microsatellite markers are effective for genetic studies in white-tailed deer.
    • These markers can be applied to other artiodactyl species.
    • Marker assignment to bovine chromosomes provides insights into conserved synteny.