Microsatellite markers in white-tailed deer
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J A DeWoody1, R L Honeycutt, L C Skow
1Department of Veterinary Anatomy and Public Health, Texas A&M University, College Station 77843, USA.
Abstract:
A genomic library of DNA from white-tailed deer was constructed for the isolation of d(AC)n microsatellite repeats. PCR primers were designed from regions flanking each repeat and used to amplify DNA samples from a pedigreed herd of white-tailed deer and other artiodactyls. Allelic frequencies, PIC values, and heterozygosity values are reported for five polymorphic markers scored in 41 animals. Homologs of two of the five markers were assigned to bovine chromosomes 4 and 23, respectively, using a panel of bovine+hamster hybrid somatic cell lines.
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