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Related Experiment Videos

Cell membrane patches are supported by proteoglycans

S P Olesen1

  • 1NeuroSearch A/S, Glostrup, Denmark.

The Journal of Membrane Biology
|April 1, 1995
PubMed
Summary

This study explored how the extracellular matrix influences membrane patch formation using patch clamp techniques. Researchers tested the effects of beta-D-xyloside, a proteoglycan synthesis inhibitor, on aortic smooth muscle and cerebellar granule cells. They found that xyloside increased the success rate of forming stable patches and led to the creation of membrane vesicles instead of inside-out patches. When amphotericin B was added to the pipette solution, perforated outside-out vesicles formed in 96% of cells. These findings suggest that the extracellular matrix or related structures support membrane patch integrity. The results imply that matrix components play a role in patch stability and morphology.

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Area of Science:

  • Cell membrane biophysics
  • Neurophysiology techniques
  • Cellular matrix interactions

Background:

Prior research has shown that cell membranes contain phospholipid bilayers and associated proteins. However, the role of the extracellular matrix in patch formation remained unclear. It was already known that proteoglycans contribute to cellular structure and signaling. No prior work had resolved how matrix components influence patch stability. This gap motivated investigations into matrix-cell membrane interactions. Some studies suggested cytoskeletal involvement in patch integrity. That uncertainty drove experiments using inhibitors to isolate matrix effects. This paper introduces a novel approach to assess matrix contributions to patch formation.

Purpose Of The Study:

The aim of this study was to examine how the extracellular matrix affects membrane patch formation. Researchers focused on proteoglycan synthesis inhibition using beta-D-xyloside. They tested this in aortic smooth muscle and cerebellar granule cells. The goal was to determine if matrix components support patch stability. The study also aimed to compare patch outcomes with and without xyloside. Another objective was to assess the impact of amphotericin B on patch formation. The researchers wanted to clarify the role of the extracellular matrix in sealing success. This work sought to provide evidence for matrix involvement in patch integrity.

Keywords:
cell membrane patchextracellular matrixpatch clampproteoglycan synthesis

Frequently Asked Questions

The study suggests that the extracellular matrix supports patch stability. Xyloside treatment altered patch morphology, indicating matrix involvement.

Beta-D-xyloside increased the seal success rate and led to vesicle formation instead of inside-out patches.

Amphotericin B was used to test vesicle formation in 96% of cells, indicating its role in patch modification.

The matrix appears to support patch stability, as its disruption altered patch morphology and increased vesicle formation.

Related Experiment Videos

Main Methods:

The study used patch clamp pipettes to isolate membrane patches from cultured cells. Aortic smooth muscle and cerebellar granule cells were selected as model systems. Beta-D-xyloside was added to inhibit proteoglycan synthesis. The xyloside was included in the cell culture medium before patching. Amphotericin B was introduced in the pipette solution to test vesicle formation. Researchers measured seal success rates and patch types formed. They compared outcomes with and without xyloside treatment. The experiments focused on quantifying patch stability and vesicle formation.

Main Results:

The xyloside increased the seal success rate in 96% of cells tested. Instead of inside-out patches, membrane vesicles formed after excision. Amphotericin B in the pipette solution produced perforated outside-out vesicles. These findings suggest a shift in patch dynamics with matrix disruption. The extracellular matrix appeared to influence patch formation outcomes. Inhibited proteoglycan synthesis altered patch morphology. The results indicate matrix components support patch integrity. These data support the hypothesis that matrix structures stabilize membrane patches.

Conclusions:

The authors propose that membrane patches are supported by extracellular matrix structures. Their findings suggest that matrix components influence patch formation dynamics. The xyloside treatment altered patch morphology, indicating matrix involvement. Amphotericin B confirmed the formation of vesicles in most cells. These results imply that matrix structures relate to patch stability. The study supports the idea that matrix integrity affects patch outcomes. The data suggest a functional link between matrix and patch formation. These conclusions align with the observed changes in patch morphology.

Vesicle formation suggests that matrix disruption changes patch dynamics and supports the role of matrix in patch integrity.

The findings suggest that matrix components influence patch formation, which may affect experimental outcomes in patch clamp studies.