Quantification of nucleolar organiser regions in canine perianal gland tumours

The proliferative activity in 28 canine perianal gland tumours was examined by the quantitative evaluation of the nucleolar organiser regions associated with argyrophil proteins (AgNORs). These regions were stained with a specific silver stain and quantified directly by light microscopy and by computerised image analysis. The relationships between some of the parameters used (the number of AgNORs per nucleus, the area of AgNORs per nucleus and the ratio of the area of AgNORs to the area of the nucleus) were also examined in relation to the histopathological grades of the tumours. All the parameters showed a high correspondence with the tumours' proliferative activity, and the ratio of the area of AgNORs to the area of the nucleus was the most significantly correlated with the tumours' histological patterns.