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Updated: Aug 19, 2026

Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification
Published on: September 15, 2012
Abstract:
It is reported on a new method of rapid cytological staining permitting valid assessment of pathological changes in the genital area, cytohormonal findings, and criteria of malignancy. The staining technique is simple, inexpensive and quick (2 minutes) and can be performed during daily routine work. The smear can immediately be evaluated and discussed with the patient. For documentation of the results the same criteria apply as in normal cytological diagnosis. Another advantage is the durability of the smears thus permitting later comparison.
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The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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