Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inducible gene targeting in mice

R Kühn1, F Schwenk, M Aguet

  • 1Institute for Genetics, University of Cologne, Germany.

Science (New York, N.Y.)
|September 8, 1995
PubMed
Summary

Researchers developed a novel gene targeting method in mice for inducible gene inactivation. This approach utilizes an interferon-responsive promoter to control Cre recombinase expression, enabling precise gene deletion in specific tissues.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Skewed B cell differentiation affects lymphoid organogenesis but not T cell-mediated autoimmunity.

Clinical and experimental immunology·2013
Same author

B cell recruitment and selection in mouse GALT germinal centers.

Current topics in microbiology and immunology·2006
Same author

Biology of Hodgkin's lymphoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2002
Same author

Regulation of immunoglobulin light chain gene rearrangements during early B cell development in the human.

European journal of immunology·2001
Same author

Receptor revision plays no major role in shaping the receptor repertoire of human memory B cells after the onset of somatic hypermutation.

European journal of immunology·2001
Same author

Homeostasis of peripheral B cells in the absence of B cell influx from the bone marrow.

The Journal of experimental medicine·2001

Area of Science:

  • Genetics
  • Molecular Biology
  • Animal Models

Background:

  • Gene targeting is crucial for studying gene function.
  • Inducible systems allow temporal control over gene inactivation.
  • Existing methods may lack tissue specificity or efficiency.

Purpose of the Study:

  • To present a new method for inducible gene inactivation in mice.
  • To demonstrate the utility of an interferon-responsive promoter for controlling Cre recombinase.
  • To validate the gene deletion efficiency in various tissues.

Main Methods:

  • Utilized an interferon-responsive promoter to drive Cre recombinase expression.
  • Employed Cre-loxP system for targeted DNA deletion.
  • Focused on the DNA polymerase beta gene as a target.
  • Administered interferon to induce gene inactivation.

Main Results:

  • Achieved complete deletion of the target gene segment in liver.
  • Demonstrated near-complete deletion in lymphocytes within days.
  • Observed partial deletion in other tested tissues.
  • Confirmed successful inducible gene inactivation in vivo.

Conclusions:

  • The developed method allows for inducible gene inactivation in mice.
  • The interferon-responsive promoter provides temporal control over gene deletion.
  • This versatile system can be applied to inactivate various target genes in vivo.

Related Experiment Videos