Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Proceedings: Experimental endocarditis. Experimental basis and prophylaxis]

L R Freedman

    Schweizerische Medizinische Wochenschrift
    |November 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Four years of the EMIC program.

    The Yale journal of biology and medicine·2010
    Same author

    [S]-AR-R 15896AR-A novel anticonvulsant: acute safety, pharmacokinetic and pharmacodynamic properties.

    The Journal of pharmacology and experimental therapeutics·1998
    Same author

    The New York High-Risk Project: attention, anhedonia and social outcome.

    Schizophrenia research·1998
    Same author

    Helping physicians recognize bedside rationing.

    Annals of internal medicine·1997
    Same author

    To prevent or not to prevent bacterial endocarditis--that is the question!

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·1993
    Same author

    Evaluation of new anti-infective drugs for the treatment of infective endocarditis. Infectious Diseases Society of America and the Food and Drug Administration.

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·1992
    Same journal

    Respiratory function test in the case of temporary phrenic nerve contusion, associated with pneumoperitoneum; About the diagnostic value of laparoscopy.

    Schweizerische medizinische Wochenschrift·2010
    Same journal

    Treatment of pulmonary tuberculosis and pleurisy by means of temporary paralysis of the diaphragm, supported by the pneumoperitoneum.

    Schweizerische medizinische Wochenschrift·2010
    Same journal

    Boeck's disease as a tuberculous syndrome.

    Schweizerische medizinische Wochenschrift·2010
    Same journal

    Enteral primary tubercular complexes

    Schweizerische medizinische Wochenschrift·2010
    Same journal

    Tuberculous stenoses of the large bronchi.

    Schweizerische medizinische Wochenschrift·2010
    Same journal

    About the enzyme action.

    Schweizerische medizinische Wochenschrift·2010
    See all related articles

    This study describes a rabbit model used to simulate human bacterial heart valve infections. Researchers found that standard antibiotic prevention methods failed in these animals, suggesting that current human clinical guidelines may require re-evaluation.

    Area of Science:

    • Infectious disease research within experimental endocarditis
    • Cardiovascular pathology and clinical pharmacology

    Background:

    Medical professionals lack a complete understanding of why certain antibiotic regimens fail to prevent heart valve infections. Prior research has shown that clinical outcomes often diverge from theoretical expectations in human patients. No prior work had resolved the specific limitations of prophylactic protocols using controlled animal models. That uncertainty drove the development of a reliable system to study these complex infections. Investigators previously struggled to replicate human disease characteristics within laboratory settings. This gap motivated the creation of a standardized procedure for inducing bacterial colonization in cardiac tissues. Scientists required a consistent platform to test the efficacy of various therapeutic interventions. The current investigation addresses these challenges by establishing a reproducible framework for evaluating infection dynamics.

    Purpose Of The Study:

    The aim of this research was to develop a simple and reliable model for producing bacterial heart valve infections. Scientists sought to overcome the difficulties associated with replicating human disease in laboratory animals. This project focused on creating a platform where the infection site could be precisely controlled. The researchers intended to evaluate whether standard human antibiotic protocols provide sufficient protection in this setting. They aimed to determine if the characteristics of the induced illness matched those seen in clinical human cases. By translating doses on a weight basis, the team examined the efficacy of current prevention strategies. This investigation was motivated by the need to improve therapeutic outcomes for patients at risk of cardiac infection. The study provides a foundation for assessing whether existing clinical recommendations require significant updates.

    Keywords:
    bacterial infectioncardiac pathologyantibiotic efficacyanimal models

    Frequently Asked Questions

    The researchers propose that inserting a polyethylene catheter into the heart cavity creates a site for bacterial attachment. Following this, intravenous injection of microorganisms consistently triggers an infection that mirrors human pathology.

    The study utilizes a polyethylene catheter placed within the venous or arterial system. This tool ensures the device tip remains positioned inside the cardiac chamber to facilitate localized colonization.

    Placement of the device tip within the heart cavity is necessary to create the specific environment required for bacterial adherence. Without this precise anatomical positioning, the subsequent injection of microorganisms fails to produce the intended disease state.

    The catheter acts as a physical substrate for bacterial attachment, which is the primary role of this component. This device allows for the controlled introduction of pathogens into the systemic circulation.

    Related Experiment Videos

    Main Methods:

    Review Approach involved evaluating a standardized rabbit model for inducing bacterial heart valve infections. Investigators inserted polyethylene tubing into the circulatory system to reach the cardiac chambers. This technique allowed for the consistent establishment of localized tissue damage. Following device placement, the team administered various microbial strains through intravenous routes. The researchers monitored the development of systemic illness to confirm successful disease induction. They compared the resulting pathological features with those documented in human clinical cases. The team also assessed the effectiveness of standard antibiotic regimens by calculating doses based on body weight. This systematic evaluation provided data on the success rates of different therapeutic interventions.

    Main Results:

    Key Findings From the Literature demonstrate that the rabbit model reliably produces infections comparable to human disease states. The researchers observed that standard prophylactic antibiotic programs were ineffective when adjusted for animal weight. This failure suggests a significant discrepancy between current clinical recommendations and actual therapeutic outcomes. The study also noted that streptococcus viridans infections in immunized subjects resulted in diffuse glomerulonephritis. These results highlight the limitations of existing prevention strategies in controlled settings. The data indicate that the model serves as a functional proxy for human infection dynamics. The team confirmed that intravenous pathogen delivery consistently leads to successful colonization of the cardiac site. These findings provide a basis for questioning the reliability of established human treatment protocols.

    Conclusions:

    Synthesis and Implications indicate that the rabbit model effectively mimics human heart valve infection characteristics. The authors suggest that weight-based antibiotic dosing fails to provide adequate protection in this experimental setup. These observations imply that current human prophylactic recommendations might be insufficient for preventing disease. The researchers propose that the efficacy of antimicrobial agents in this model likely mirrors human responses. Consequently, the team argues for a critical re-evaluation of existing clinical guidelines for treatment. This review highlights the potential risks associated with relying on outdated prevention strategies. Future clinical practices should consider the limitations identified through these animal-based trials. The findings emphasize the necessity of aligning therapeutic protocols with rigorous experimental evidence.

    The researchers observed that streptococcus viridans infections in immunized animals led to diffuse glomerulonephritis. This measurement demonstrates the systemic impact of the induced cardiac infection on renal health.

    The authors propose that because antibiotic efficacy in rabbits likely reflects human responses, current clinical recommendations for prophylaxis require urgent reconsideration. They emphasize that standard dosing regimens proved ineffective during their trials.