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Production of brefeldin-A
T G McCloud1, M P Burns, F D Majadly
1Chemical Synthesis and Analysis Laboratory, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
Journal of Industrial Microbiology
|July 1, 1995
Summary
Researchers optimized fermentation conditions to produce the antitumor antibiotic, brefeldin-A, using Eupenicillium brefeldianum. A new HPLC method enabled efficient monitoring, leading to the isolation of one kilogram of brefeldin-A.
Area of Science:
- Microbiology
- Biotechnology
- Pharmacology
Background:
- Eupenicillium brefeldianum produces the antitumor antibiotic brefeldin-A.
- Efficient production and isolation methods are crucial for therapeutic applications.
Purpose of the Study:
- To describe fermentation conditions for brefeldin-A production.
- To develop a rapid analytical method for brefeldin-A quantitation.
- To achieve large-scale isolation of brefeldin-A.
Main Methods:
- Liquid culture fermentation of Eupenicillium brefeldianum (ATCC 58665).
- Development of an analytical High-Performance Liquid Chromatography (HPLC) method.
- Large-scale isolation from fermentation broth.
Main Results:
- Optimized fermentation conditions for brefeldin-A production were established.
- A rapid HPLC method was successfully developed for real-time monitoring.
- One kilogram of brefeldin-A was isolated from a 6800-liter fermentation.
Conclusions:
- The study successfully optimized brefeldin-A production and isolation.
- The developed HPLC method facilitates efficient fermentation monitoring.
- Large-scale production of brefeldin-A is feasible for potential therapeutic use.