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Cytokine-induced release of plasminogen activator inhibitor-1 by human mesothelial cells
1Department of Surgery, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
Objective:
To see what effect the inflammatory cytokines tumour necrosis factor (TNF), interleukin 1 (IL-1), and interleukin 6 (IL-6) had, both alone and in combination, on the production of plasminogen activator inhibitor 1 (PAI-1) by human mesothelial cells.
Design:
Laboratory study.
Setting:
University hospital, UK.
Material:
Six cell cultures of human mesothelial cells.
Main Outcome Measures:
Concentrations of PAI-1 in conditioned media and cell lysates after culture for 24 hours.
Results:
TNF, IL-1, and IL-6 all significantly increased the amount of PAI-1 in the media compared with controls (81%, p < 0.05; 77%, p < 0.05; and 60%, p < 0.05, respectively). TNF and IL-1 together produced significantly more release than each cytokine alone (190%, p < 0.05) and a combination of the three increased release even further (290%, p < 0.05). The presence of endotoxin did not significantly affect the release of PAI-1.
Conclusion:
Inflammatory cytokines mediate the release of PAI-1 by mesothelial cells, and so may affect the deposition of fibrin within the peritoneal cavity.