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Decrease of NCAM expression and astrocyte-neurone interaction in long-term cultured astrocytes

T Yazaki1, H Asou, K Arimoto

  • 1Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.

Neuroreport
|May 30, 1995
PubMed
Summary
This summary is machine-generated.

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Older astrocytes show reduced neural cell adhesion molecule (NCAM) and increased laminin expression, impairing neuron interaction. These changes in protein expression impact the aging nervous system.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Aging Research

Background:

  • Astrocytes play crucial roles in nervous system development and aging.
  • Understanding astrocyte characteristics is vital for studying neurodegenerative processes.

Purpose of the Study:

  • To investigate the protein expression profile of aged astrocytes.
  • To assess the relationship between astrocyte aging and neuronal interaction.

Main Methods:

  • Utilized long-term cultured rat astrocytes (20 months) and short-term controls.
  • Employed immunocytochemical and ELISA assays to analyze protein expression.
  • Evaluated astrocyte-neurone interaction capabilities.

Main Results:

  • Long-term astrocytes exhibited decreased neural cell adhesion molecule (NCAM) expression.

Related Experiment Videos

  • Laminin expression was significantly increased in aged astrocytes.
  • Aged astrocytes showed reduced interaction with neurons compared to young astrocytes.
  • Astrocytes remained positive for glial fibrillary acidic protein (GFAP).

    • Conclusions:

    • Changes in NCAM and laminin expression characterize aged astrocytes.
    • These molecular alterations correlate with diminished astrocyte-neurone communication.
    • NCAM may be a key molecule involved in nervous system development and aging.