Related Experiment Video
Updated: Aug 17, 2026

Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
Published on: July 13, 2011
Antioxidant properties of nitecapone are potentiated by glutathione
E Nissinen1, I B Lindén, P Pohto
1Orion-Farmos, Orion Research, Espoo, Finland.
Abstract:
The effect of nitecapone (3[(3,4-dihydroxy-5-nitrophenyl) methylene]-2,4-pentanedione) on lipid peroxidation in guinea pig liver microsomes was assessed with the thiobarbituric acid assay. Nitecapone inhibited dose-dependently hydroxyl and peroxyl radical induced lipid peroxidation with IC50-values of 11.1 microM and 16.2 microM, respectively. Nitecapone was an effective antioxidant in native microsomes, where it potentiated glutathione-dependent protection against oxidative stress. Nitecapone provided dose-dependent reduction in lipid peroxidation lasting up to three hours in the presence of glutathione. The long-lasting effect was lost, when the microsomes were boiled. These data suggest that nitecapone is an effective scavenger of oxygen derived free radicals, and that its antioxidant properties are potentiated by glutathione.
More Related Videos
11:58Synthesis and Functionalization of Nitrogen-doped Carbon Nanotube Cups with Gold Nanoparticles as Cork Stoppers
Published on: May 13, 2013
09:02Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
Published on: June 9, 2017
Related Concept Videos
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
2° Amines to N-Nitrosamines: Reaction with NaNO2
Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
Phase II Reactions: Acetylation Reactions
The substrates for acetylation are typically drugs or their metabolites with an amino, sulfonamide, or hydrazine functional group. Acetylation can occur at several points in the drug molecule, including primary, secondary, and...
Bioactivation and Tissue Toxicity