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[Comparative characteristics of PBV-1 and PBV-3 viruses isolated from Penicillium breve-compactum]
Abstract:
PBV-1 and PBV-3 viruses isolated from mycelium of Penicillium brevi-compactum show similar morphology and practically the same size of virus particles. Both viruses have been shown to lyse very effectively E. coli C, K12, W3350, CR63. Reproduction of the viruses in E. coli CK cells is limited due to the phenomenon of modification by the host, and negative colonies formed by each on E. coli CK sheet differ in morphology. PBV-1 virus is highly infectious for E. coli B, while the capacity of PBV-3 to multiply in these cells is very low. The limited reproduction appears to be explained by modification of PBV-3 DNA in the E. coli B system. PBV-1 and PBV-3 viruses have been shown to differ serologically, although serological cross reactions indicate their antigenic relationship. The two viruses under study differ in the constituency and molecular weight of the main structural proteins: 36,000: 28,000: 26,500 and 17,000 daltons for PBV-1 and 39,000; 36,000; 32,500; 31,000 and 18,000 daltons for PBV-2.
Insights
Penicillium brevi-compactum viruses (PBV-1 and PBV-3) effectively lyse E. coli strains but show varied infectivity due to host modification. These related viruses differ in protein structure and serology.
Area of Science:
- Virology
- Microbiology
- Molecular Biology
Background:
- Penicillium brevi-compactum viruses (PBV-1 and PBV-3) are mycoviruses.
- These viruses exhibit similar morphology and particle size.
Purpose of the Study:
- To characterize and compare PBV-1 and PBV-3 viruses.
- To investigate their lytic activity and reproductive behavior in different E. coli strains.
- To analyze their serological relationship and protein composition.
Main Methods:
- Isolation and characterization of PBV-1 and PBV-3.
- Lytic activity assays on various E. coli strains.
- Host modification studies in E. coli CK and E. coli B.
- Serological analysis and SDS-PAGE for protein profiling.
Main Results:
- Both PBV-1 and PBV-3 effectively lyse E. coli strains C, K12, W3350, and CR63.
- Reproduction in E. coli CK is limited by host modification, resulting in distinct colony morphologies.
- PBV-1 shows high infectivity in E. coli B, while PBV-3 exhibits very low infectivity, attributed to DNA modification.
- PBV-1 and PBV-3 are serologically related but distinct, with differing major structural protein molecular weights.
Conclusions:
- PBV-1 and PBV-3 are closely related but distinct mycoviruses with differential host interactions.
- Host modification plays a significant role in regulating virus replication and infectivity.
- Structural protein differences contribute to the observed variations between PBV-1 and PBV-3.