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Related Experiment Videos

Connective tissue mast cells exhibit time-dependent degranulation heterogeneity

M S Kaminer1, G F Murphy, B Zweiman

  • 1Department of Dermatology, University of Pennsylvania, Philadelphia, USA.

Clinical and Diagnostic Laboratory Immunology
|May 1, 1995
PubMed
Summary

Mast cells (MC) exhibit two degranulation patterns during allergic reactions. Rapid anaphylactic degranulation occurs alongside slower, gradual changes in other granules within the same cell, suggesting granule heterogeneity.

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Area of Science:

  • Immunology
  • Cell Biology
  • Allergy Research

Background:

  • Mast cells (MC) release mediators through distinct degranulation processes.
  • Anaphylactic degranulation is rapid, involving granule fusion and mediator release.
  • Piecemeal degranulation is slower, with gradual mediator release via microvesicular transport.

Purpose of the Study:

  • To investigate the mechanisms and temporal dynamics of mast cell degranulation patterns.
  • To determine if degranulation differences are due to stimulus type, granule heterogeneity, or timing.
  • To examine mast cell responses in immunoglobulin E (IgE)-mediated allergic reactions.

Main Methods:

  • Electron microscopic examination of skin biopsies from ragweed-sensitive subjects.
  • Biopsies were collected at various time points (15s to 10 min) after intradermal ragweed injection.

Related Experiment Videos

  • Analysis focused on ultrastructural changes in mast cell granules.

    • Main Results:

    • Anaphylactic-type granule changes initiated within 15 seconds and were complete by 5 minutes.
    • Both swollen/fused and unaffected granules were observed concurrently within the same mast cells.
    • Later granule alterations resembled piecemeal degranulation, but without observed microvesicular transport.

    Conclusions:

    • Skin mast cells in IgE-mediated reactions display both rapid anaphylactic and slower, gradual degranulation.
    • These distinct patterns can occur simultaneously within a single mast cell.
    • Findings suggest mast cell granule heterogeneity may influence responses to IgE-mediated stimuli.