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Updated: Oct 12, 2026

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
12:22

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

Expression of Pax-2 in human renal cell carcinoma and growth inhibition by antisense oligonucleotides

J R Gnarra1, G R Dressler

  • 1Urological Oncology Section, National Cancer Institute, Bethesda, Maryland 20892, USA.

Cancer Research
|September 15, 1995
PubMed

Insights

Pax-2 gene expression is common in renal cell carcinoma (RCC) and is crucial for tumor cell proliferation. Inhibiting Pax-2 may offer a novel therapeutic strategy for treating kidney cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Renal cell carcinoma (RCC) is the most prevalent adult kidney malignancy.
  • RCC is believed to originate from the proximal tubule epithelium.
  • Pax-2 is essential for renal epithelium development.

Purpose of the Study:

  • To investigate the expression of Pax-2 in renal cell carcinoma.
  • To determine the role of Pax-2 in RCC cell proliferation.
  • To explore Pax-2 as a potential therapeutic target for RCC.

Main Methods:

  • Immunohistochemical staining of primary tumor samples.
  • Analysis of Pax-2 protein and mRNA expression in RCC cell lines.
  • Treatment of RCC cell lines with antisense oligodeoxynucleotides.

Main Results:

  • Pax-2 was expressed in malignant RCC cells, not in surrounding stroma.
  • 73% of human RCC cell lines showed Pax-2 protein and mRNA expression.
  • Antisense oligodeoxynucleotides targeting Pax-2 reduced protein levels and inhibited cell growth.

Conclusions:

  • Pax-2 gene function is vital for RCC cell proliferation.
  • Pax-2 plays a role in both embryonic renal development and RCC progression.
  • Targeting Pax-2 presents a potential novel therapeutic approach for renal cell carcinoma.

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